cell lines mh s atcc cat  (ATCC)

Journal: Cells

Article Title: Exosomal microRNAs from Alveolar Macrophages Reveal a Protective Role of the Lung Microbiome Against Oncogenic Signaling During PAH Exposure

doi: 10.3390/cells15080715

Figure Lengend Snippet: Characterization of small extracellular vesicles (sEV) isolated from variously treated cultured murine alveolar macrophages (MH-S cell line). Left panels ( A – H ): Size distribution plots from nanoparticle tracking analysis according to particle diameter. ( A ) Vehicle-only treated MH-S cells. ( B ) Microbiome-only treated MH-S. ( C ) B[a]P-only treated MH-S (low concentration; 1 μg/mL). ( D ) B[a]P-only treated MH-S (high concentration; 10 μg/mL). ( E ) Microbiome + B[a]P (1 μg/mL). ( F ) Microbiome + B[a]P (10 μg/mL). ( G ) Microbiome + B[a]P (1 μg/mL) in the presence of AHR antagonist CH223191 . ( H ) Microbiome + B[a]P (10 μg/mL) in the presence of AHR antagonist CH223191 . Right panels ( a – h ) Representative transmission electron microscopy (TEM; 200,000×) images of sEVs isolated from each respective treatment group are presented in the right panel of each plot; arrows highlight representative sEVs. A 100 nm scale bar is provided in the bottom right-hand corner of each image for perspective. Abbreviations; Conc. (concentration).

Article Snippet: Murine alveolar macrophage cell line MH-S (CRL-2019), purchased from American Type Culture Collection (ATCC), (Manassas, VA, USA) and maintained as described elsewhere [ ], was used in this study as a source of cultured alveolar macrophages (AMs).

Techniques: Isolation, Cell Culture, Concentration Assay, Transmission Assay, Electron Microscopy

Journal: Cells

Article Title: Exosomal microRNAs from Alveolar Macrophages Reveal a Protective Role of the Lung Microbiome Against Oncogenic Signaling During PAH Exposure

doi: 10.3390/cells15080715

Figure Lengend Snippet: Expression of the extracellular vesicle protein markers CD9 (top panel) and TSG101 (bottom panel) in small extracellular vesicles (sEVs) isolated from the culture medium of different treatment groups. Lane 1: Vehicle-only treated MH-S cells. Lane 2: Microbiome-only treated MH-S. Lane 3: B[a]P-only treated MH-S (low concentration; 1 μg/mL). Lane 4: B[a]P-only treated MH-S (high concentration; 10 μg/mL). Lane 5: Negative control (PBS only). Lane 6: Positive control. Lane 7: Microbiome + B[a]P (1 μg/mL). Lane 8: Microbiome + B[a]P (10 μg/mL). Lane 9: Microbiome + B[a]P (1 μg/mL) in the presence of AHR antagonist CH223191 . Lane 10: Microbiome + B[a]P (10 μg/mL) in the presence of AHR antagonist CH223191 . Lane 11: Negative control (PBS only). Lane 12: Positive control.

Article Snippet: Murine alveolar macrophage cell line MH-S (CRL-2019), purchased from American Type Culture Collection (ATCC), (Manassas, VA, USA) and maintained as described elsewhere [ ], was used in this study as a source of cultured alveolar macrophages (AMs).

Techniques: Expressing, Isolation, Concentration Assay, Negative Control, Positive Control

Journal: Particle and Fibre Toxicology

Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor

doi: 10.1186/s12989-026-00674-9

Figure Lengend Snippet: Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of Calcein-AM (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure

Article Snippet: The murine alveolar macrophage cell line MH-S cells were obtained from Procell (Wuhan, China).

Techniques: CCK-8 Assay, Control, Fluorescence, Staining

Journal: Particle and Fibre Toxicology

Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor

doi: 10.1186/s12989-026-00674-9

Figure Lengend Snippet: ACAFP-induced oxidative stress and mitochondrial dysfunction in MH-S cells. A Representative fluorescence images of intracellular ROS levels detected by DCFH-DA (green). B Representative fluorescence images of mitochondrial membrane potential assessed by TMRE (red). Quantification of C DCFH-DA, and D TMRE fluorescence intensity. Nuclei, blue; * Compared to control group, p < 0.05; # Compared to inside 300 µg/mL ACAFP group, p < 0.05

Article Snippet: The murine alveolar macrophage cell line MH-S cells were obtained from Procell (Wuhan, China).

Techniques: Fluorescence, Membrane, Control

Journal: Particle and Fibre Toxicology

Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor

doi: 10.1186/s12989-026-00674-9

Figure Lengend Snippet: NLRP3 inflammasome activation and pyroptosis induction in ACAFP-exposed macrophages. A Expression of NLRP3, ASC, GSDMD (full-length and N-terminal fragment), and IL-1β (pro-form and mature form) in MH-S cells exposed to different concentrations of ACAFP for 24 h. B Concentration of IL-1β in cell culture supernatant measured by ELISA. C Expression of pyroptosis-related proteins in MH-S cells pre-treated with or without MCC950 for 1 h followed by exposure to 300 µg/mL inside or outside ACAFP for 24 h. Quantitative analyses of D cell viability, E percentage of PI-positive cells, F LDH release, and G IL-1β concentration in supernatant under the indicated interventions. * p < 0.05

Article Snippet: The murine alveolar macrophage cell line MH-S cells were obtained from Procell (Wuhan, China).

Techniques: Activation Assay, Expressing, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay